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Table 2 Stability of RNA transcription of reference genes in fluconazole-treated C. glabrata as determined by the 2 -ΔΔCT method

From: Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

  RDN18 RDN25 RDN5.8 UBC13 PGK1 UBC7 GAPDH UBC4 TUB1 EF1α PPIA RPL2A RPL10 RPL13A ACT1 SDHA
C T Change 0.34 0.39 0.46 1.34 1.56 1.60 2.17 2.41 2.60 2.89 3.13 3.67 4.12 4.17 4.45 5.03
ΔΔC T (t) 0.12 0.07 0 -0.87 -1.10 -1.13 -1.71 -1.95 -2.13 -2.43 -2.66 -3.21 -3.65 -3.71 -3.98 -4.57
2 -ΔΔCT (t) 0.92# 0.95# 1.00 1.83* 2.14* 2.19* 3.28* 3.86* 4.39* 5.38* 6.32* 9.23* 12.58* 13.06* 15.82* 23.75*
Ranking 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
  1. CT Change = CT(ut) − CT(t); ut, untreated; t, fluconazole-treated.
  2. ΔCT = CTreference − CTRDN5.8; ΔCT of each reference gene was calculated using RDN5.8 as the internal control.
  3. ΔΔCT(t) = ΔCT(t) − ΔCT(ut).
  4. 2-ΔΔCT(t) for fluconazole-treated cells indicates fold change in RNA transcription of a reference gene normalized to RDN5.8, as compared with untreated cells.
  5. The stability ranking is based on the values of CT Change, ΔΔCT(t), and 2-ΔΔCT(t) of the reference genes in fluconazole-treated C. glabrata cells.
  6. #P > 0.05 and *P <; 0.05 for the azole-treated group vs. the untreated group after normalizing to RDN5.8.