Interaction of C/EBP-beta and NF-Y factors constrains activity levels of the nutritionally controlled promoter IA expressing the acetyl-CoA carboxylase-alpha gene in cattle

Table 1 Relative mRNA levels of ACC-α PIA, NF-YA and C/EBPβ in various tissues under different nutritional conditions

	Copies	Liver		Adipose		Mammary gland
Diet		Straw	Normal	Fat	Starch	Starch	Fat
ACC-α PIA	x 10⁴	0.10 ± 0.01 ^a	0.52 ± 0.22 ^b	6.81 ± 2.24	8.49 ± 4.86	19.79 ± 4.79	42.85 ± 12.98
NF-YA	x 10³	8.94 ± 1.05	7.64 ± 0.71	10.41 ± 0.97	18.57 ± 7.61	8.38 ± 1.48	7.56 ± 1.06
C/EBPβ	x 10⁶	4.37 ± 0.25	3.71 ± 0.80	2.45 ± 0.35	3.08 ± 1.10	0.62 ± 0.09^a	0.36 ± 0.06 ^b
NFYA:C/EBPβ	x 10^-3	2.06 ± 0.24	2.44 ± 0.64	4.69 ± 0.71	6.45 ± 0.90	14.07 ± 1.87^a	25.89 ± 5.08 ^b
NEL	MJ	18.0 ± 1.1	110.2 ± 11.2	nd	nd	nd	nd
Fat	g/kg DM	nd	nd	51	28	28	51
Carbohydrate	g/kg DM	nd	nd	31	100	100	31
n	6	6	8	8	8	8	8

Mean values (±S.E.M.). Different superscripts (“a” and “b”) indicate statistically different values (P < 0.05) within the respective tissue. NEL, Net Energy content for Lactation; nd, not determined; n, number of animal.

ISSN: 1471-2199