Skip to main content
Figure 7 | BMC Molecular Biology

Figure 7

From: Characterization of 3'-untranslated region of the mouse GDNF gene

Figure 7

ARE in the mouse GDNF 3'-UTR destabilized luciferase mRNA in C6 cells. Thirty-six hours after transfection with the indicated reporter construct, together with the pEGFP-N1 vector, the cells were harvested (0 h, untreated cells) or treated with Act-D (5 μg/ml) for 1 or 3 h. The total RNA isolated from each sample was subjected to RT-PCR, and the relative mRNA levels of luciferase and EGFP were calculated by a comparison of β-actin or EGFP-normalized values with the level in the untreated cells (0 h) as described in the Materials and Methods (A, B). Values represent the means ± SD from four independent cultures.

Back to article page