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Figure 5 | BMC Molecular Biology

Figure 5

From: Characterization of 3'-untranslated region of the mouse GDNF gene

Figure 5

ARE in the mouse GDNF 3'-UTR down-regulated luciferase mRNA in C6 cells. A) Thirty-six hours after transfection with each GDNF promoter construct containing the indicated length of the 3'-UTR, the luciferase activity was measured as described in the Materials and Methods. Values represent the means ± SD from 3 independent cultures and are expressed relative to the luciferase activity of the pGL3-Promoter vector (pGL3pro). Δ8R represents a reporter construct in which the Δ8 fragment of the mouse GDNF 3'-UTR was inserted in the opposite direction. B) Thirty-six hours after transfection with the indicated reporter constructs, total RNA from each sample was extracted and subjected to RT-PCR as described in the Materials and Methods. The relative mRNA level of luciferase was calculated by the comparison to β-actin-normalized values of the mRNA level in pGL3-Promoter vector-transfected cells. Values represent the means ± SD from 3 independent cultures and are expressed relative to the luciferase activity (A) and mRNA (B) of the pGL3-Promoter vector. *p < 0.01, compared to the luciferase activity in the pGL3-Promoter vector-transfected cells (pGL3pro).

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