Strain | Viability |
---|
BY4741 | 95,33 ± 8,08 |
Δyhr087w
| 73,67 ± 0,58 |
cdc33
ts
| 41,71 ± 8,33 |
Δyhr087w cdc33
ts
| 27,34 ± 3,45 |
- Dilutions from cultures in exponential phase of growth were plated on YPD or YPD + hygromycin 10 μg/mL plates and the colony forming units appeared 48-72 hours later were counted. Viability was determined as the percentage between the CFU in hygromycin plates and in YPD plates. Application of the t-test to the data obtained for cdc33ts and Δyhr087w cdc33ts mutant strains gave a P value (calculated for one tail using Microsoft Office Excel) of 0.0025.