Figure 7

Sensitivity to translation elongation inhibitors of the mutants in YHR087W and/or other genes encoding proteins that physically interact with Yhr087wp (according to the TAP experiment) and are involved, most of them, in the ribosomal function: STI1 , SCP160 , TIF4631 (Panel A) and CDC33 (Panel B). Serial dilutions were spotted on YPD, YPD cycloheximide (0.1 μg/mL) and YPD hygromycin (20 μg/mL or 10 μg/mL) plates, which were incubated for 48-72 h at 30°C. Incubation with mutants cdc33^ts and Δyhr087w cdc33^ts was carried out at 33°C until growth was detected.