Figure 2

Regulation of the YHR087W gene expression by the HOG pathway, the Msn2/4p, Hot1p and Sko1p transcription factors, and the Bcy1p subunit of the PKA. Cells from the exponential cultures at 30°C in YPD medium of several wild type and mutant strains were maintained under these conditions (control, C), transferred to YP20 for 30 min (20%) or were affected by a 30-min heat shock at 39°C (39°C). Then were collected and total RNA was isolated. Gene expression was determined by Northern analysis using a specific probe. Data have been normalized with the SRC1 gene. Images (panel A) show a representative example of the results obtained. Quantification of some of these results (panel B) has been carried out from the data obtained in three independent experiments; the average value and the standard deviation appear. In this quantification, the mRNA level found in each mutant under each stress condition is represented as the percentage of that detected in the corresponding wild type strain in the same situation, which is considered 100.