Skip to main content

Advertisement

Table 1 Plasmids encoding N- or C-terminal fragments of M.SssI

From: Complementation between inactive fragments of SssI DNA methyltransferase

Plasmid Res. N-terminal extension1 C-terminal extension1
pB-Sss[1–189] Ap   EI
pBNH-Sss[1–176] Ap MVPGMH6LEC KLGCFGG
pBNH-Sss[1–222] Ap MVPGMH6LEC ASLAVLADERRFSA
pBNH-Sss[1–239] Ap MVPGMH6LEC SCFGG
pBNH-Sss[1–248] Ap MVPGMH6LEC LAVLADERRFSA
pBNH-Sss[1–257] Ap MVPGMH6LEC EAWLFWRMREDFQPDTD
pBNH-Sss[1–304] Ap MVPGMH6LEC  
pBNH-Sss[1–317] Ap MVPGMH6LEC KLGCFGG
pBNH-Sss[1–318] Ap MVPGMH6LEC KLGCFGG
pBNH-Sss[1–324] Ap MVPGMH6LEC LGCFGG
pBNH-Sss[1–356] Ap MVPGMH6LEC KLGCFGG
pB6ZB-Sss[1–239] Ap 6-ZFP-B zinc finger domain SCFGG
pB6ZB-Sss[1–304] Ap 6-ZFP-B zinc finger domain  
pOB-Sss[191–386] Kn MVP SH6C
pOB-Sss[231–386] Kn MVP SH6C
pOB-Sss[240–386] Kn MV SH6C
pOB-Sss[241–386] Kn M SH6C
pOB-Sss[242–386] Kn MVQ SH6C
pOB-Sss[246–386] Kn MV SH6C
pOB-Sss[250–386] Kn MVH SH6C
pOB-Sss[254–386] Kn MVP SH6C
pOB-Sss[261–386] Kn MV SH6C
pOB-Sss[279–386] Kn M SH6C
pOB-Sss[304–386] Kn M SH6C
pOB-Sss[316–386] Kn MVP SH6C
pOB-Sss[321–386] Kn MVP SH6C
pSss[191–386]del2 Kn MVP SH6C
pSss[241–386]del2 Kn M SH6C
pOB-Sss[191–386]SS Kn   SH6C
pOB-Sss[241–386]-6ZA Kn M 6-ZFP-A zinc finger domain
pOB-Sss[261–386]-6ZA Kn MV 6-ZFP-A zinc finger domain
pBS-CAL75 Ap   
pBS-Sss6ZA Ap   6-ZFP-A zinc finger domain
  1. Numbers in square brackets specify the M.SssI fragment expressed by the plasmid.
  2. 1 N- and C-terminal extensions were added on purpose (His tag) or are byproducts of the cloning procedure. 2Plasmid lacking the P BAD promoter.