Figure 5

mRNA expression of enzymes and co-factors involved in DNA demethylation in unstimulated CD4⁺ T cells in two age groups of mice. Total RNA was prepared from unstimulated CD4⁺ T cells in both age groups of mice. After reverse transcription, quantitative PCR analysis was performed on cDNA with primers designed to detect these enzymes and co-factors. mRNA expression of (A) DNA methyltransferases (Dnmt1, Dnmt3a), (B) deaminases (Apobec3 and Apobec4), glycosylases (Mbd4 and Tdg), Gadd45 family (Gadd45a, Gadd45b and Gadd45g) and (c) ten-eleven translocation family (Tet1, Tet2 and Tet3) was determined by real-time PCR. The gene expression data were normalized to the Ubc housekeeping gene, and are shown as the mean and SEM of three independent experiments