Figure 1

Global DNA methylation and chromosomal accessibility in CD4⁺ T cells. A. - C. Genomic DNA isolated from total CD4⁺ T cells taken from the spleens of 2 week or 16 week old C57BL/6 mice that were either unstimulated (NS) or stimulated with PMA/I for 4 hours (A), FACS-sorted naive T cells from both age groups (B), or FACS-sorted naive T cells and nTreg cells or differentiated Th1, Th2, Th17, and iTreg cells (C) were digested with McrBC for 6 hours at 37°C and the DNA fragments were resolved on a 1% agarose gel. Uncut genomic DNA is also shown. D. & E. Nuclei isolated from CD4⁺ T cells of 2 weeks (D) and 16 weeks (E) old C57BL/6 mice were digested with various concentrations of MNase (10 U to 120 U) at 37°C for 5 min, prior to loading onto a 1% agarose gel for separation. The DNA was visualised by ethidium bromide staining in all experiments. The 1 kb plus ladder is indicated by the letter L. Every experiment was repeated at least 3 times