Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities

Table 2 Bioinformatics analysis of TspDTI domains and functional motifs

Residues	domain	HHPRED	PCONS score	predicted function
1 ~ 160	PD-(D/E)XK	1m0d (Endo I) 21	1avq (λ exonuclease) 0.18	Mg²⁺-binding/DNA cleavage
~160-360	helical linker	no match	no match	interactions between domains
~360-790	RFM	2ar0 (M.EcoKI) 4.6e-36	2adm (M.TaqI) 1.9	SAM-binding/methylation
~790-	TRD	1ih2 (M.TaqI) 1.9e-13	1yf2 (S.MjaORF132P) 0.374	DNA-binding

ISSN: 1471-2199