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Figure 7 | BMC Molecular Biology

Figure 7

From: Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities

Figure 7

Effect of allosteric cofactors on TspDTI REase activity. 1 μg (= 5.58-pmol restriction sites) bacteriophage λ DNA was digested with 2.79 pmol TspDTI (0.5:1 M ratio of enzyme to recognition sites) for 30 min at 70°C and electrophoresed on 1.2% agarose/TBE gel. Lane M1, 1 kb ladder (selected bands marked); lane M2, 100 bp ladder (selected bands marked); lane K, undigested λ DNA; lane 1, (+ TspDTI, no allosteric cofactor; lane 2, (+ TspDTI, + 50 μM SIN); lane 3, (+ TspDTI, + 50 μM SAM); lane 4, (+ TspDT, + 50 μM SAH); lane 5, (+ TspDTI, + 50 μM ATP).

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