High recovery of cell-free methylated DNA based on a rapid bisulfite-treatment protocol

BMC Molecular Biology

Table 2 Detection of methylated DNA diluted in unmethylated DNA

Copies of methylated DNA	0	1.3	4	11.9	35.6	107	320	960
Detected methylated copies (RASSF1A)	0	0.5	4.9	11	41	142	328	832
Detected unmethylated copies (RASSF1A)	1748	2192	2297	2207	1832	2700	2285	2194

Detection of RASSF1A in a 3-fold dilution series of methylated DNA in a background of 2000 copies of DNA. Top row represents the calculated number of methylated copies of RASSF1A promoter. The following rows represent methylated and unmethylated copies respectively, detected by real-time PCR.

ISSN: 1471-2199