Figure 9

Effects on the MTG16 promoter activity of GATA-1 siRNA. Transfection was as described in Methods. The relative mRNA expression of GATA-1 (black bar) and MTG16 (white bar) is given for HEL cells (wt), HEL cells transfected with non silencing scrambled control siRNA (control si) and HEL cells transfected with siRNA for GATA-1 (GATA-1 si). The protein expression of GATA-1 and MTG16 are shown with GAPDH as acontrol of equal loading. The experiments were performed in triplicate. Bars represent the mean and the error bars show SEM.