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Table 3 Effects of plant SPO11 expression on X chromosome nondisjunction in the Drosophila mei-W681 homozygote

From: The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay

Transgene of mei-W681homozygote

Mothers crossed

Total progeny scored

Nullo-X eggs

(%)

Diplo-X eggs

(%)

Nullo-X eggs without associated diplo-X eggs

(%)

P value from chi test

Exp. 1

      

None

99

1283

146 (11.4)

106 (8.3)

40 (3.1)

-

AtSPO11-1 a

74

1204

138 (11.5)

95 (7.9)

43 (3.6)

5.3E-01

AtSPO11-2 b

71

1187

178 (15.0)

88(7.4)

90 ** (7.6)

6.9E-07

OsSPO11D c

97

1384

184 (13.3)

106 (7.7)

78 ** (5.6)

1.6E-03

Exp. 2

      

none

55

1245

119 (9.6)

88 (7.1)

31 (2.5)

-

OsSPO11D Y213Fd

57

1994

175 (8.8)

129 (6.5)

46 (2.3)

7.4E-01

mei-W68 + e

22

3434

3 (0.09)

3 (0.09)

0 (0)

-

  1. Every female (w/w) was crossed to a few males of Canton-S strain (w+ /Y). Nullo-X and diplo-X eggs were detected as w+ sons (X0) and as w daughters (XXY), respectively. The number of nullo-X eggs without associated diplo-X eggs was calculated by subtracting the number of diplo-X eggs from the number of nullo-X eggs. The number of diplo-X eggs was used as the expected value for the number of nullo-X eggs with associated diplo-X eggs, which were generated by chromosome nondisjunction during the first meiotic division. The egg laying periods were 5 days and 10 days in Exp. 1 and Exp. 2, respectively. Transgenes are indicated as Vector name{gene, insertion #}.
  2. a: P{hsp83-AtSPO11-1 cDNA, A5-1}
  3. b: P{hsp83-AtSPO11-2 cDNA, 2M3-1}
  4. c: P{hsp83-OsSPO11D cDNA, F2-1}
  5. d: P{hsp83-OsSPO11DY213F, F22-F2}
  6. e: P{hsp83-mei-W68+ cDNA, M53-3}
  7. ***: P < 1E-03; **: 1E-03 <P < 1E-02; *: 1E-02 <P < 5E-02.