The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay

Table 2 Effects of plant SPO11 expression on fertility in the Drosophila mei-W68¹(dmspo11- deficient) homozygote

mei-W68¹hetero- zygosity	Transgene	Mothers crossed	Total eggs laid	Pupae generated	% Fertility
+/1	none	37	3768	3309	88
1/1	none	107	6109	1568	26
1/1	mei-W68 ^{+ §}	11	1207	1047	87
1/1	AtSPO11-1 ^a	40	2880	691	24
1/1	AtSPO11-2 ^b	20	1536	359	23
1/1	OsSPO11D ^c	60	3577	753	21

Every female (w/w) was crossed to a few males of Canton-S strain (w⁺ /Y). The numbers of total eggs laid and the pupae subsequently generated were scored. Fertility was calculated by dividing the number of pupae (representing viable eggs) by the number of total eggs. Transgenes are indicated as Vector name{gene, insertion #}.
§: P{hsp83-mei-W68⁺ cDNA, M53-3}
a: P{hsp83-AtSPO11-1 cDNA, A5-1}
b: P{hsp83-AtSPO11-2 cDNA, 2M3-1}
c: P{hsp83-OsSPO11D cDNA, F2-1}

ISSN: 1471-2199