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Table 1 Relative levels of mRNA from copies of the plant SPO11 transgene in Drosophila ovaries

From: The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay

Insertions of transgenes

Relative levels of mRNA*

P{hsp83-AtSPO11-1 cDNA, A5-1}

3.40 ± 0.40

P{hsp83-AtSPO11-2 cDNA, 2M3-1}

9.22 ± 2.72

P{hsp83-OsSPO11A cDNA, 3M2-3}

13.88 ± 3.59

P{hsp83-OsSPO11B cDNA, M6-2}

19.85 ± 4.50

P{hsp83-OsSPO11B cDNA, F3-2}

14.57 ± 6.32

P{hsp83-OsSPO11D cDNA, F2-1}

3.17 ± 0.36

P{hsp83-OsSPO11D Y213F , F22-F2}

4.22 ± 0.84

P{hsp83-OsSPO11D Y213F , M33-F1}

3.76 ± 1.39

  1. * The amount of mRNA from each copy of the plant SPO11 transgene in Drosophila ovaries was normalized by the amount of mRNA from the RP49 gene. The average value with SD for each of the relative levels of transcripts was obtained from three samples, each containing 5~10 ovaries.
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BMC Molecular Biology

ISSN: 1471-2199

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