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Table 2 Details of PCR amplification of a genomic DNA template.

From: Assessment and validation of a suite of reverse transcription-quantitative PCR reference genes for analyses of density-dependent behavioural plasticity in the Australian plague locust

Symbol size
(bp)
Tm
(°C)
Cq
(cycles)
18SrRNA ~170 84.3 14.8
Arm no amplification no amplification no amplification
EF1a # light smear 3 peaks: 76.6; 79.8; 83.7 35.8
RpL32 ~100 78.4 28.8
GAPDH # light smear 79.8 34.9
Actin ~650 no amplification no amplification
SDHa no amplification no amplification no amplification
AnnIX light smear no amplification no amplification
  1. Genomic DNA amplicons were sized on an agarose gel following 30 cycles of PCR amplification using a Taq polymerase from Qiagen, a melting temperature of 58°C, and 50 ng of template. The melting temperatures (Tm) and Cq values were determined by quantitative PCR on a LightCycler 480 Instrument (Roche) as detailed in the main text and on 5 ng of genomic DNA. # means that primers span an exon-exon boundary. Alignments of complementary and genomic DNA sequences can be found for Actin, GAPDH, and EF1a in Additional file 3.