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Table 1 Primers used in this study
| Primers | Primer sequence |
|---|---|
| Degenerate primers | |
| Nl FoxAF1 | 5-CACGCSAAGCCBCCVTACTC-3 |
| Nl FoxAF2 | 5-ATCACSATGGCBATMCAGAA-3 |
| Nl FoxAR1 | 5-TCYTTYTTYTCGTCYTTGAA-3 |
| Nl FoxAR2 | 5-CCGTTYTCRAACATGTTVCC-3 |
| For RACE | |
| 3Nl FoxA1 | 5'-AGTTTCAATGACTGTTTTGTGAA-3' |
| 3Nl FoxA2 | 5'-TTCTGGACSCTGCATCCCGAGT-3' |
| 5Nl FoxA1 | 5'-AGAATTCTGGATRGCCATCG-3' |
| 5Nl FoxA2 | 5'-GATARGTCTCTGATAGTGTGA-3' |
| For RT-PCR and real-time PCR | |
| Q Nl FoxAF | 5'-TTACACGCACGCTAAACCAC-3' |
| Q Nl FoxAR | 5'-CCTCATCAGCCCAAGGGAACAA-3' |
| Q Nl VgF | 5'-GCATCAATGAACCCAGCTAACTC-3' |
| Q Nl VgR | 5'-TGGACGGCTCTTTGCATACTCC-3' |
| ACTIN-F | 5'-TGCGTGACATCAAGGAGAAGC-3' |
| ACTIN-R | 5'-CGGCACCTTCACAAAACAG-3' |
| For Nl FoxA dsRNA synthesis | |
| Nl FoxA -F | 5'-TTCGAGAACGGCTGCTACCTGCGG-3' |
| Nl FoxA -R | 5'-GCTCTCGGCGGGCAGCAGCC-3' |
| For GFP dsRNA synthesis | |
| GFP-F | 5'-AAGGGCGAGGAGCTGTTCACCG-3' |
| GFP-R | 5'-CAGCAGGACCATGTGATCGCGC-3' |
| For Nl Vg protein expression | |
| Nl Vg-EF | 5'-GTA CCATGG CCAGTAACT TCCCCAATG TG-3' |
| Nl Vg-ER | 5'-GAT CTCGAG CTTGGCCAAGACAACAACCTTC-3' |
| For Nl Vg dsRNA synthesis | |
| Nl Vg -F | 5'-ACAGCCAGTCCAACAGCTTCTAC-3' |
| Nl Vg-R | 5'-TGCTGCTGCTGCTGCTGCTTC-3' |
