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Degenerate primers
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Nl FoxAF1
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5-CACGCSAAGCCBCCVTACTC-3
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Nl FoxAF2
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5-ATCACSATGGCBATMCAGAA-3
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Nl FoxAR1
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5-TCYTTYTTYTCGTCYTTGAA-3
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Nl FoxAR2
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5-CCGTTYTCRAACATGTTVCC-3
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For RACE
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3Nl FoxA1
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5'-AGTTTCAATGACTGTTTTGTGAA-3'
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3Nl FoxA2
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5'-TTCTGGACSCTGCATCCCGAGT-3'
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5Nl FoxA1
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5'-AGAATTCTGGATRGCCATCG-3'
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5Nl FoxA2
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5'-GATARGTCTCTGATAGTGTGA-3'
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For RT-PCR and real-time PCR
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Q Nl FoxAF
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5'-TTACACGCACGCTAAACCAC-3'
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Q Nl FoxAR
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5'-CCTCATCAGCCCAAGGGAACAA-3'
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Q Nl VgF
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5'-GCATCAATGAACCCAGCTAACTC-3'
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Q Nl VgR
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5'-TGGACGGCTCTTTGCATACTCC-3'
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ACTIN-F
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5'-TGCGTGACATCAAGGAGAAGC-3'
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ACTIN-R
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5'-CGGCACCTTCACAAAACAG-3'
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For Nl FoxA dsRNA synthesis
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Nl FoxA -F
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5'-TTCGAGAACGGCTGCTACCTGCGG-3'
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Nl FoxA -R
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5'-GCTCTCGGCGGGCAGCAGCC-3'
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For GFP dsRNA synthesis
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GFP-F
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5'-AAGGGCGAGGAGCTGTTCACCG-3'
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GFP-R
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5'-CAGCAGGACCATGTGATCGCGC-3'
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For Nl Vg protein expression
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Nl Vg-EF
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5'-GTA CCATGG CCAGTAACT TCCCCAATG TG-3'
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Nl Vg-ER
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5'-GAT CTCGAG CTTGGCCAAGACAACAACCTTC-3'
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For Nl Vg dsRNA synthesis
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Nl Vg -F
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5'-ACAGCCAGTCCAACAGCTTCTAC-3'
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Nl Vg-R
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5'-TGCTGCTGCTGCTGCTGCTTC-3'
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