Table 1 Primers used in this study
Primers | Primer sequence |
|---|---|
Degenerate primers | |
Nl FoxAF1 | 5-CACGCSAAGCCBCCVTACTC-3 |
Nl FoxAF2 | 5-ATCACSATGGCBATMCAGAA-3 |
Nl FoxAR1 | 5-TCYTTYTTYTCGTCYTTGAA-3 |
Nl FoxAR2 | 5-CCGTTYTCRAACATGTTVCC-3 |
For RACE | |
3Nl FoxA1 | 5'-AGTTTCAATGACTGTTTTGTGAA-3' |
3Nl FoxA2 | 5'-TTCTGGACSCTGCATCCCGAGT-3' |
5Nl FoxA1 | 5'-AGAATTCTGGATRGCCATCG-3' |
5Nl FoxA2 | 5'-GATARGTCTCTGATAGTGTGA-3' |
For RT-PCR and real-time PCR | |
Q Nl FoxAF | 5'-TTACACGCACGCTAAACCAC-3' |
Q Nl FoxAR | 5'-CCTCATCAGCCCAAGGGAACAA-3' |
Q Nl VgF | 5'-GCATCAATGAACCCAGCTAACTC-3' |
Q Nl VgR | 5'-TGGACGGCTCTTTGCATACTCC-3' |
ACTIN-F | 5'-TGCGTGACATCAAGGAGAAGC-3' |
ACTIN-R | 5'-CGGCACCTTCACAAAACAG-3' |
For Nl FoxA dsRNA synthesis | |
Nl FoxA -F | 5'-TTCGAGAACGGCTGCTACCTGCGG-3' |
Nl FoxA -R | 5'-GCTCTCGGCGGGCAGCAGCC-3' |
For GFP dsRNA synthesis | |
GFP-F | 5'-AAGGGCGAGGAGCTGTTCACCG-3' |
GFP-R | 5'-CAGCAGGACCATGTGATCGCGC-3' |
For Nl Vg protein expression | |
Nl Vg-EF | 5'-GTA CCATGG CCAGTAACT TCCCCAATG TG-3' |
Nl Vg-ER | 5'-GAT CTCGAG CTTGGCCAAGACAACAACCTTC-3' |
For Nl Vg dsRNA synthesis | |
Nl Vg -F | 5'-ACAGCCAGTCCAACAGCTTCTAC-3' |
Nl Vg-R | 5'-TGCTGCTGCTGCTGCTGCTTC-3' |