Differential expression level of Vg proteins in ovariesof BPH. (A) ELISA analysis. PBST (0.15 M, pH7.4) was used as the blank control. (1) CK, Control, (2) GFP, 0.5 μg/μl ds GFP, (3) ds Nl FoxA, 0.5 μg/μl ds NlFoxA. Data are means ± S.E.M of three independent experiments with fifteen individuals each. '*' means statistically significant difference in OD450 values between CK and ds NlFoxA (t-test, p < 0.05). (B) Western Blot analysis. Immunoblotted with anti-Vg serum (diluted 1:5000) and visualized by ECL. Actin was used as an internal control.