Detection of the efficiency of feeding-based RNAi and impact on NlFoxA mRNA level by RT-qPCR. The expression levels of N. lugens FoxA mRNA after different treatments. CK, control; dsGFP, 0.5 μg/μlds GFP; ds Nl FoxA, 0.5 μg/μl ds NlFoxA, respectively. The data represent the mean values ± S.E.M of three replicates. '*'means statistically significant difference in expression levels between CK and ds Nl FoxA(t-test, p < 0.05).