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Table 1 Oligonucleotides used for the cloning of different fragments of Dkl2 promoter in pGL3Basic and pGL3promoter vectors

From: Characterization of a proximal Sp1 response element in the mouse Dlk2 gene promoter

DNA FRAGMENT OLIGONUCLEOTIDES SEQUENCE RESTRICTION ENZYMES
-1,090/+1* Dlk2MluI-917U 5'-ATTACGCGTTTGTCAGGTGTAGGCGGTGGG-3' Mlu I-Hind III
  Dlk2HindIII-1L 5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'  
-910/+1* Dlk2MluI-722U 5'-GGCGACGCGTCAAATACACATATTGGGGTCTT-3' Mlu I-Hind III
  Dlk2HindIII-1L 5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'  
-710/+1* Dlk2MluI-522U 5'-TATACGCGTGGCAGGCTACCCAAAGGTGG-3' Mlu I-Hind III
  Dlk2HindIII-1L 5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'  
-575/+1* Dlk2MluI-400U 5'-TAGACGCGTAAGAAGCCCACAGAGAGCAGGC-3' Mlu I-Hind III
  Dlk2HindIII-1L 5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'  
-375/+1* Dlk2MluI-205U 5'-TATACGCGTTGGGTGAGGGGCAGAGTGG-3' Mlu I-Hind III
  Dlk2HindIII-1L 5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'  
-212/+1* Dlk2 Xho-212U 5'-TATCTCGAGGAAGGGAGGGGCGAAGAGC-3' Xho I-Hind III
  Dlk2HindIII-1L 5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'  
-1,090/-375 Dlk2MluI-917U 5'-ATTACGCGTTTGTCAGGTGTAGGCGGTGGG-3' Mlu I-Hind III
  Dlk2HindIII-184L 5'-GCTAAGCTT CCACTCTGCCCCTCACCCAC-3'  
-1,090/-575 Dlk2MluI-917U 5'-ATTACGCGTTTGTCAGGTGTAGGCGGTGGG-3' Mlu I-Hind III
  Dlk2HindIII-379L 5'-TAGAAGCTTCGCCTGCTCTCTGTGGGCTTC-3'  
-910/-375 Dlk2MluI-722U 5'-GGCGACGCGTCAAATACACATATTGGGGTCTT-3' Mlu I-Hind III
  Dlk2HindIII-184L 5'-GCTAAGCTT CCACTCTGCCCCTCACCCAC-3'  
-710/-375 Dlk2MluI-522U 5'-TATACGCGTGGCAGGCTACCCAAAGGTGG-3' Mlu I-Hind III
  Dlk2HindIII-184L 5'-GCTAAGCTT CCACTCTGCCCCTCACCCAC-3'  
+1/+177 Dlk2Xho I+1U 5'-ATTCTCGAGCCCAGATTCCCGAGTGCTCGGC-3' Xho I-Hind III
  Dlk2HindIII+177L 5'-ATGAAGCTTAGGGACCGCGTCCTCCTAGCTTC-3'  
-212/+177* Dlk2 Xho-212U 5'-TATCTCGAGGAAGGGAGGGGCGAAGAGC-3' Xho I-Hind III
  Dlk2HindIII+177L 5'-ATGAAGCTTAGGGACCGCGTCCTCCTAGCTTC-3'  
-212/-427* Dlk2 Xho-212U 5'-TATCTCGAGGAAGGGAGGGGCGAAGAGC-3 Xho I-Hind III
  Dlk2HindIII+427L 5'-AATAAGCTT GTGGCCACCGCGCGGGAAC-3  
  1. PCR reactions were performed under standard conditions (see Materials and Methods), except where indicated with an asterisk, in which the reactions were supplemented with 10% DMSO.