STE12 and STE11ΔN activation of FKS2 promoter in mating and HOG pathway mutants. GAL1prom-STE12 over expressed in presence of FKS2 (-928 to +1)-lacZ (A), FKS2 (-706 to +1)-lacZ (B) and Δ-178 pCYC1-lacZ (C). GAL1prom-STE11ΔN over expressed in presence of FKS2 (-928 to +1)-lacZ (D), FKS2 (-706 to +1)-lacZ (E), PRM5-lacZ (F) and Δ-178 pCYC1-lacZ (G) and FKS2(-928 to -706)-lacZ (H). Strains were pregrown in raffinose- dextrose medium and then in galactose medium for 6 hours at 30°C. Data were expressed as mean ± SE of at least three independent experiments. Statistical significance was computed by the unpaired Student's t test. *p < 0.05, **p < 0.01.