Figure 3
From: Francisella RNA polymerase contains a heterodimer of non-identical α subunits
Transcription activity of in vitro assembled Francisella RNAP core enzymes. A. The structure of nucleic acid scaffold used to test assembled enzymes activities is schematically shown. B. Superose 6 fractions obtained after separation of in vitro RNAP assembly reactions containing indicated subunits were combined with nucleic acid scaffold containing a radioactively labelled RNA primer and reactions were supplemented with NTP. Reaction products were analyzed by denaturing PAGE. An autoradiograph is presented.