Figure 1From: Francisella RNA polymerase contains a heterodimer of non-identical α subunitsIn vitro assembly of Francisella α homo- and heterodimers. Reactions containing indicated proteins were combined at denaturing conditions and, following dialysis into a buffer favouring RNAP assembly, were fractionated using Ni2+-affinity chromatography. Coomassie-stained SDS gels are presented. "L" - load, "F" - flow-through, "E" - elution with buffers with indicated concentration of imidazole.Back to article page