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Figure 1 | BMC Molecular Biology

Figure 1

From: Francisella RNA polymerase contains a heterodimer of non-identical α subunits

Figure 1

In vitro assembly of Francisella α homo- and heterodimers. Reactions containing indicated proteins were combined at denaturing conditions and, following dialysis into a buffer favouring RNAP assembly, were fractionated using Ni2+-affinity chromatography. Coomassie-stained SDS gels are presented. "L" - load, "F" - flow-through, "E" - elution with buffers with indicated concentration of imidazole.

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