Evaluation of suitable reference genes for gene expression studies in bronchoalveolar lavage cells from horses with inflammatory airway disease

Table 2 Primers information and PCR reactions efficiencies for candidate reference genes.

Gene	Accession Number	Oligo	Sequence	Amplicon size (bp)	Efficiency ± SD (%)
ACTB	AF035774	Forward	CTGGCACCACACCTTCTACA	249	96.15 ± 5.74
		Reverse	CCCTCATAGATGGGCACAGT
GAPDH	AF083897	Forward	GGTGAAGGTCGGAGTAAACG	106	96.25 ± 4.63
		Reverse	AATGAAGGGGTCATTGATGG
B2M	X69083	Forward	CCTGCTCGGGCTACTCTC	89	103.45 ± 5.54
		Reverse	CATTCTCTGCTGGGTGACG
HPRT	AY372182	Forward	AATTATGGACAGGACTGAACGG	121	101.05 ± 3.27
		Reverse	ATAATCCAGCAGGTCAGCAAAG
RPL32	CX594263	Forward	GGGAGCAATAAGAAAACGAAGC	138	101.25 ± 2.45
		Reverse	CTTGGAGGAGACATTGTGAGC
SDHA	DQ402987	Forward	GAGGAATGGTCTGGAATACTG	91	100.5 ± 1.96
		Reverse	GCCTCTGCTCCATAAATCG
UBB	AF506969	Forward	TTCGTGAAGACCCTGACC	91	100.65 ± 2.35
		Reverse	CCTTATCCTGGATCTTGGC

Primers sequences were determined using primer3 software and Ensembl Genome Browser (EMBL accession numbers are indicated). Efficiencies are averages of all individual efficiencies calculated for each reaction using LinReqPCR software.

ISSN: 1471-2199