Figure 1

Diagram of the microplate-based Matrix ChIP-MeDIP method. Batches of polypropylene plates are coated overnight with protein A (room temperature) and are then stored sealed up to 2 weeks (4°C) and used when needed. Starting with protein A-coated plates the ChIP-MeDIP assay takes 4-5 hours to generate PCR-ready DNA. Detailed protocol is described in "Method" section. The red and yellow lines exemplify rows of wells with ChIP and MeDIP antibodies, respectively.