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Table 2 Quantitative protein analysis of the immunized haemolymph of S. littoralis and the purified defensin peptide after induction of the recombinant E. coli by IPTG.

From: Identification, phylogenetic analysis and expression profile of an anionic insect defensin gene, with antibacterial activity, from bacterial-challenged cotton leafworm, Spodoptera littoralis

Protein concentration at different hours post-infection (μg/ml)
Mean ± S.E.
  1 h 2 h 3 h 6 h 12 h 24 h 48 h 72 h
Cont-H 588 ± 6.1 - - 624.8 ± 6.4 620.2 ± 4.8 651.4 ± 7.5 645.4 ± 5.3 675.6 ± 2.5
Inf-H 687.8 ± 8.9 - - 908.2 ± 7.2 919.4 ± 4.7 948.8 ± 6.2 2017.6 ± 9.5 1316.4 ± 13.0
Expec. A.B. 99.8 - - 283.4 299.2 297.4 1372.1 640.8
Induced 909 ± 4.9 1057.2 ± 30.5 1413.2 ± 38.9 - - - - -
NonInd 702.2 ± 6.0 725.8 ± 6.4 938.8 ± 14.1 - - - -  
Expressed P. 206.8 ± 3.8 331.4 ± 24.4 474.4 ± 36.2      
Purified 141 ± 7.8 163.8 ± 6.4 414 ± 4.0 - - - - -
Loss 65.8 ± 0.5 167.6 ± 1.3 60.4 ± 0.2      
df, F, P 4, 1694.2, 0.0 4, 458.1, 0.0 4, 397.9, 0.0 4, -, 0.0 4, -, 0.0 4, -, 0.0 4, -, 0.0 4, -, 0.0
  1. Cont-H: Untreated crude haemolymph.
  2. Inf-H: Treated haemolymph.
  3. Expec.: Expected antibacterial peptide concentration.
  4. Induced: IPTG induced E. coli culture.
  5. NonInd: Non-induced E. coli culture.
  6. Expressed P.: (Induced - Non-induced).
  7. Purified: Purified expressed peptide using Ni-affinity column.
  8. Loss: Loss due to purification.