Table 2 Quantitative protein analysis of the immunized haemolymph of S. littoralis and the purified defensin peptide after induction of the recombinant E. coli by IPTG.
Protein concentration at different hours post-infection (μg/ml) Mean ± S.E. | ||||||||
|---|---|---|---|---|---|---|---|---|
| Â | 1 h | 2 h | 3 h | 6 h | 12 h | 24 h | 48 h | 72 h |
Cont-H | 588 ± 6.1 | - | - | 624.8 ± 6.4 | 620.2 ± 4.8 | 651.4 ± 7.5 | 645.4 ± 5.3 | 675.6 ± 2.5 |
Inf-H | 687.8 ± 8.9 | - | - | 908.2 ± 7.2 | 919.4 ± 4.7 | 948.8 ± 6.2 | 2017.6 ± 9.5 | 1316.4 ± 13.0 |
Expec. A.B. | 99.8 | - | - | 283.4 | 299.2 | 297.4 | 1372.1 | 640.8 |
Induced | 909 ± 4.9 | 1057.2 ± 30.5 | 1413.2 ± 38.9 | - | - | - | - | - |
NonInd | 702.2 ± 6.0 | 725.8 ± 6.4 | 938.8 ± 14.1 | - | - | - | - |  |
Expressed P. | 206.8 ± 3.8 | 331.4 ± 24.4 | 474.4 ± 36.2 |  |  |  |  |  |
Purified | 141 ± 7.8 | 163.8 ± 6.4 | 414 ± 4.0 | - | - | - | - | - |
Loss | 65.8 ± 0.5 | 167.6 ± 1.3 | 60.4 ± 0.2 |  |  |  |  |  |
df, F, P | 4, 1694.2, 0.0 | 4, 458.1, 0.0 | 4, 397.9, 0.0 | 4, -, 0.0 | 4, -, 0.0 | 4, -, 0.0 | 4, -, 0.0 | 4, -, 0.0 |