Table 1 Key table for the primers used in DD-PCR study providing their names, origin and sequences.
Primer name | Origin | Sequence (5' -- 3') |
|---|---|---|
CHIF | Chitinase-based | TGCCTTTGATTCAGTCATC |
CHIR | Chitinase-based | AATAATCGACTCCAATACG |
EGF | Endoglucanase-based | TCCGGGTATGTTATGGAAGA |
EGR | Endoglucanase-based | GGCCATCCACTCTCAGACACA |
LECF | Lectin-based | ATGGGATCCAAGCAACAGAG |
LECR | Lectin-based | ATCCTTCAAAGACACAATGTCG |
IDF | Insect defensin-based | CCAAATGCCTCGTCATCT |
IDR | Insect defensin-based | ATTAGAGTCAAGCTAAAAGGG |
HDF | Human defensin-based | TTATTTCTTTCTTCGGCAGC |
HDR | Human defensin-based | GGAGCCCTTTCTGAATCCGCA |
FLDefF | Full length defensin | GTTCGTCTATTTTTGTGCCG |
FLDefR | Full length defensin | ACTTAAAAATCTATCATTGGCGTCA |
MPDefF | Mature peptide defensin | GTTTCATGCGATTTCGAGGAAGCC |
MPDefR | Mature peptide defensin | TCATGTGTAGGTATTTGTGTACC |
PromF | Random primer | GGTCCCTGACTGATCCCTGG |
PromR | Flanking region of cDNA | CTCATTATTTATATAACCTTAAC |