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Figure 4 | BMC Molecular Biology

Figure 4

From: Cis-regulatory functions of overlapping HIF-1alpha/E-box/AP-1-like sequences of CD164

Figure 4

Effects of hypoxia on CD164 expression. (A) Hypoxia increased activity of CD164 promoter. HCT 116 cells were transfected with pGL3P4 plasmids and treated in hypoxia chamber. The normalized luciferase activities were shown. After 8 hours, the activity of pGL3P4 in hypoxia group was significantly higher than that of normal group (p < 0.05 student's t-test). No significant difference was found at 1hr and 2hrs. (B) HIF-1 alpha protein level was tested using western blot and there was an increase of HIF-1alpha protein expression in a time-dependent manner. (C) Real-time PCR was performed to investigate the upregulated expression CD164 at mRNA level in HCT116 after transfection of HIF-1 α. (D) Chromatin immunoprecipitation analysis of HIF-1alpha binding to the CD164 promoter. DNA released from the precipitated complexes was amplified by PCR. The PCR products were separated by agarose gel electrophoresis. Lane 1: IgG control; Lane 2: anti-RNA polymerase II; Lane 3: HIF-1alpha antibody pull-down; Lane 3:Input

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