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Table 3 Relative amounts of BR in Hbt. salinarum WT R1 cells and brz S9, brb S9, brz S9brb S9, bp2 stop and bp4 stop mutants

From: A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

 

Dark

Light

ID

OD600 = 0.7-0.8

OD600 = 1.1-1.2

OD600 = 0.7-0.8

OD600 = 1.1-1.2

WT

1.20

2.95

1.13

3.16

brz S9

0.00

0.18

0.00

0.06

brb S9

1.32

3.07

1.43

3.58

brz S9brb S9

0.27

1.22

0.32

1.59

bp2 stop

1.75

2.92

1.34

3.32

bp4 stop

1.13

3.09

1.38

3.25

  1. For BR measurement cell samples were taken at the same time as for northern blot analysis. BR concentration values are given in μM.
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BMC Molecular Biology

ISSN: 1471-2199

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