A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

Table 2 Comparison of effects of the brz and brb mutations on bop transcription and BR production at OD₆₀₀ = 1.1-1.2

Mutant ID	State of Brz	State of Brb	Effect on bop	Effect on BR amount
			mRNA level	Dark	Light
brb CBD	WT	fusion brb-CBD	down	down	down
brb stop1	WT	1 stop codon in brb	WT	WT	WT
brb stop2	WT	2 stop codons in brb	WT	WT	WT
brz S9	mutation S9	WT	down	down	down
brb S9	WT	mutation S9	WT	WT	WT
brz S9brb S9	mutation S9	mutation S9	WT	1/2 of WT	1/2 of WT
brb M1	WT	mutation M1	WT	WT	WT
brz S9brb M2	mutation S9	mutation M2	down	down	down

Cell samples were taken at the same time for northern blot analysis and BR measurement.

ISSN: 1471-2199