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Table 1 Relative amounts of BR in Hbt. salinarum WT R1 cells and brb CBD, brb stop1, brb stop2, brb M1, brz S9brb M2 mutants

From: A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

 

Dark

Light

ID

OD600 = 0.7-0.8

OD600 = 1.1-1.2

OD600 = 0.7-0.8

OD600 = 1.1-1.2

WT R1

1.20

4.54

1.60

3.94

brb CBD

0.20

1.18

0.25

1.00

brb stop1

0.66

3.78

1.16

3.46

brb stop2

1.24

3.96

1.29

3.91

brb M1

1.50

5.01

1.36

4.47

brz S9brb M2

0.09

0.95

0.00

0.64

  1. For BR measurement cell samples were taken at the same time as for northern blot analysis. BR concentration values are given in μM.
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BMC Molecular Biology

ISSN: 1471-2199

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