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Figure 2 | BMC Molecular Biology

Figure 2

From: A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

Figure 2

Test of brb translation start codon activity. Panel A represents the organization of the brb and bgaH genes in the chromosome of Hbt. salinarum after integration of suicide plasmids pVYT1 and pVYT2 (additional file 9). Panel B shows colonies of a strain expressing an active Brb-BgaH fusion protein (insertion of pVYT1) on plates containing X-gal (40 μg ml-1). Panel C presents a control mutant lacking β-galactosidase activity as a result of elimination of the brb start codon in the brb-bgaH gene fusion (insertion of pVYT2).

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