293 cells were transfected with Nef/Stop and Nef/WT plasmids for 36 h. Total RNA was isolated and processed for gene array as described in Experimental Procedures. Five genes whose expression was significantly inhibited by Nef were identified to be either known retinoic acid-inducible genes or contained RAR/RXR binding sites in their promoters. IFIT1, CEBPB, and CRABP2 were found to be known retinoic acid-inducible genes whereas. RBM8A and ACOT2 were found to contain putative RARE (DR2) and RXRE (DR1) sites respectively in their promoter regions upstream of the transcription start site. The inhibition of gene expression was validated by Real-Time RT-PCR.