Table 1 Primer sequences and amplicon characteristics of DCLs, XTH, and Gh-miR162
Gene | Forward primer sequence (5'-3') | Reverse primer sequence (5'-3') | Amplicon size (bp) | Efficiency ± SD* | R2 | Locus accession number |
|---|---|---|---|---|---|---|
DCL1 | AACCCTGGGTGGTGTCCCCTG | ATGCCCCCTTTTGGCTGGCTC | 132 | 0.928647 ± 0.0293724 | 0.9858 | ES804646.1 |
DCL2 | GATCGCTATCATGCTTCTCCGCAG | TGGGGAACCAAGAAGACAGCGAA | 81 | 0.99522 ± 0.00361129 | 0.9903 | DW488144 |
DCL3 | ATGTCCACATGCCCCCTGAGCT | GGCCAACATTAAGGACTCCAGCCG | 113 | 0.997162 ± 0.0071986 | 0.9646 | DR462994 |
DCL4 | GCTTCCAAGCGGCAACAGCATT | AGGATGCACAATCGCCTGAAGGAG | 186 | 0.992603 ± 0.0051324 | 0.9706 | DT568872 |
Gh-miR162 | GCGGCGGAGCTATTTGGAGACG | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACCTGGAT | 72 | 0.884982 ± 0.032819 | 0.8519 | - |
XTH | GGAAAGGGTGACAGGGAACA | GGCTGGAGTTTTGGGTATGG | 173 and 392** | - | - | AY189971.2 |