Quantitative RT-PCR analysis. Expression levels of Gh-miR162 (A) and cotton Dcl mRNAs (B) in infected and uninfected plants. Relative expression levels were estimated using polyubiquitin and catalytic subunit of protein phosphatase 2A genes as internal controls. Error bars indicate standard deviations. P values were calculated by t-tests with a significance level of P < 0,05. Asterisks indicate significant differences.