Figure 5
From: Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription
Effects of wild-type and Rasd1 mutants on nuclear-cytoplasmic distribution of Ear2. (A-G) Immunofluorescence staining of COS-7 cells transfected with pHisHA-Rasd1 (A1-3) or pGST-Ear2 (B1-3) or pHisHA-Rasd1 (wild type or mutants) + pGST-Ear2 (C1 to G4). HisHARasd1 (wild type or mutants) were detected with anti-HA antibody and visualized with AlexaFluor 568 (red); GST-Ear2 was labeled with anti-GST AlexaFluor 488 (green); nucleus was labeled with 4',6-diamidino-2-phenylindole (DAPI) (blue). Confocal imaging showed that HisHA-Rasd1 is present in both the nucleus and cytoplasm (A1-3). GST-Ear2 is located mainly in the nucleus (B1-3), but when co-transfected together with HisHA-Rasd1, GST-Ear2 is detected in both the nucleus and cytoplasm (C2).