Table 3 Human TMEM16A transcript scanning assay
From: Increased complexity of Tmem16a/Anoctamin 1 transcript alternative splicing
Primer Set | Oligonucleotide sequences (5'→ 3') | Tmem16a region | Size (bp) | Alternative exon(s) identified |
|---|---|---|---|---|
A | ACAGGCGGCCACGATGAGGGTC (sense) GAGCCTGCATCCAGCGACGCC (antisense) | Exons 1-2 | 348 | Â |
B | AGGAGGGTGCAGCACAGCGA (sense) ATTGGCCAGCAGGCTCGTGA (antisense) | Exons 2-7 | 582 648 | + Exon 6b |
C | GAGAACGACGTGTACAAAGGCCAAG (sense) | Exons 6-16 | 714 | + Exon 13 |
| Â | TTAGTGAGGTAGGCTGGGAACCGAT (antisense) | Â | 780 | + Exons 6b + 13 |
| Â | Â | Â | 792 | + Exons 13 + 15 |
| Â | Â | Â | 858 | + Exons 6b +13 + 15 |
| Â | Â | Â | 579 | - Exons 10 + 13 |
| Â | Â | Â | 657 | - Exons 10 + 13+15 |
| Â | Â | Â | 708 | + Exons 6b + 13 - 14 |
D | ACTGACAAAGTGAAGCTGACA (sense) | Exons 16-20 | 526 | Â |
| Â | TGTTCTGGATCAGCTGTTTC (antisense) | Â | 414 | - Exon 18 |
E | TTTCCGTTCCTTCCGAATGG (sense) | Exons 19-23 | 460 | Â |
| Â | CTTCCCAATGCCTCTGAGGATA (antisense) | Â | Â | Â |
F | TGTCACTGAGCTCCGAAGGCCG (sense) | Exons 22-25 | 384 | Â |
| Â | ACGATGACAAACGCCAGCCGG (antisense) | Â | Â | Â |
G | AGTAAGAACGGGACCATGCACGG (sense) | Exons 24-26 | 509 | Â |
| Â | CGCTGGCATAGCTACAGGACGC (antisense) | Â | Â | Â |