Table 2 Mouse Tmem16a transcript scanning assay
From: Increased complexity of Tmem16a/Anoctamin 1 transcript alternative splicing
Primer Set | Oligonucleotid sequences (5'→ 3') | Tmem16a region | Size (bp) | Alternative exon(s) identified |
|---|---|---|---|---|
A | GAGGGAACCTCTGCGGACCGA (sense) | Exons 1-3 | 613 | Â |
|  | GCCAGGGCGCATGGATCTTC (antisense) |  | 610 | Δ3' Exon 1 |
B | GGCCTGGAGTTGGAGAATGACG (sense) | Exons 2-10 | 602 | Â |
| Â | GTGTAGGCTCCAAGCCAGGCAA (antisense) | Â | 668 | + Exon 6b |
C | GAACAACGTGCACCAAGGCCAAGTA (sense) | Exons 6-16 | 699 | Â |
| Â | TGGTGAAATAGGCTGGGAATCGGTC (antisense) | Â | 711 | + Exon 13 |
| Â | Â | Â | 777 | + Exon 15 |
| Â | Â | Â | 777 | + Exons 6b + 13 |
| Â | Â | Â | 789 | + Exons 13 + 15 |
| Â | Â | Â | 819 | + Exon 13b |
| Â | Â | Â | 654 | - Exons 10 +13 +15 |
| Â | Â | Â | 705 | - Exons 6b +13 -14 |
D | TGGAGCACTGGAAACGGAAG (sense) | Exons 12-20 | 625 | Â |
| Â | ATACAGAGCTCCATGAGGCAGC (antisense) | Â | 637 | + Exon 13 |
| Â | Â | Â | 715 | Exons 13 + 15 |
E | ACCGACAAGGTGAAGCTGAC (sense) | Exons 16-22 | 731 | Â |
| Â | GACGCAACAAACAGGGTGAC (antisense) | Â | 619 | - Exon 18 |
|  |  |  | 623 | Δ3' Exon 21 |
F | GAAGTTTACGGCTGCATTGC (sense) | Exons 17-24 | 719 | Â |
| Â | ATGTAGAGGTACACCAGGCGAG (antisense) | Â | Â | Â |
G | GCCTCATGGAGCTCTGTATCC (sense) | Exons 20-26 | 767 | Â |
| Â | CACCCAGTCCACAAAGTCACTC (antisense) | Â | Â | Â |
H | TGGTATAACATCCTCAGAGGTG (sense) | Exons 23-26 | 656 | Â |
| Â | TCACTTGGTGATGGTCAGAA (antisense) | Â | Â | Â |