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Figure 4 | BMC Molecular Biology

Figure 4

From: Increased complexity of Tmem16a/Anoctamin 1 transcript alternative splicing

Figure 4

Identification of a novel exon 13b in Tmem16a. A. Cartoon representation of the novel exon 13b identified through sequence analysis of a Tmem16a fragment amplified using primers that span exons 6 through 16. Exon13b (120 bp) is highlighted. The genomic region between exon 13 and 14 is expanded to indicate the location of exon 13b, the splice and branch sites and the polypyrimidine tract. B. Alignment of the 40 amino acids encoded by exon 13b of mouse with the corresponding residues identified in rat, human and rhesus monkey. C. RT-PCR with primer sets 5'Exon13b and 3'Exon13b verified the expression of exon 13b in mouse heart. Cartoon of the amplification products generated with these primers is shown. Primer set 5'Exon13b produced two fragments, the 290 bp fragment consisted of exons 11, 12 and 13b; the 463 bp fragment consisted of exons 11, 12, 13 and13b plus the entire intronic sequence between exon 13 and 13b. The 3'E13b primer set yielded three fragments. The 219 bp and 297 bp fragments included exons 12, 13b, 14, ± 15, 16, and the 492 bp fragment consisted of 12, 13b, 14, -15, 16 plus the intronic sequence between exon 13b and 14. The intron retention events are highlighted.

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