Figure 2

Multiple Tmem16a variants are expressed in heart due to different combinations of alternative exons 6b, 13 and 15. A. Cartoon indicating eight possible Tmem16a transcript variants as a result of combinations of exon 6b, 13 or 15 inclusion and/or exclusion. Dashed lines denote the exclusion of an alternative exon. Primers which span Tmem16a from exon 6 to exon 16, a region encompassing the three alternative exons (shaded), generate transcripts of various sizes depending upon which exons are amplified. The expected sizes of RT-PCR products are indicated. Tmem16a variant [GenBank: NM_178642] lacking all 3 exons is indicated (#). B. Gel analysis of Tmem16a RT-PCR fragments ranging between 657 and 855 bp were amplified. 100 bp ladder (L).