Patterns of GFP expression driven by different GAL4 lines. (A) UAS-97 bp/GAL4-twi.2xPE 3-4 h old embryo; (B) whole ovary dissected from an UAS-97 bp; GAL4::VP16-nos.UTR female. The arrows indicate the germarium; (C) whole ovary from an UAS-97 bp female, note the absence of GFP expression at the tip of the ovary where the germarium is located (arrows); (D) dissected ovariole from an UAS-97 bp; GAL4::VP16-nos.UTR female; (E) dissected UAS-97 bp; Lsp2-GAL4 third instar larvae showing GFP expression in the larval fat body; (F, G) dissected fat body and associated organs from an UAS-97 bp; Lsp2-GAL4 early prepupae, note that GFP expression is only verified in the fat body; (H) dissected UAS-97 bp/GAL4-GMR larva, the inset shows a dissected antenna-eye imaginal disc from a larva of the same genotype, note that GFP expression occurs only in the eye imaginal disc; (I) dissected UAS-GFP.S65T/GAL4-GMR larva showing GFP expression both in the eye disc and in the salivary gland; (J) dissected UAS-97 bp; pPTGAL26 larva, note the presence of a low frequency of GFP positive cells in the salivary glands; (K) dissected UAS-GFP.S65T; pPTGAL26 larva, note GFP expression in the salivary glands. (A) confocal microscopy; (A') bright field image of the same embryo as shown in (A), (B, C, E, F, G, H, I, J, K) fluorescence stereoscopy; (D) overlay between confocal microscopy and bright field. (rg) ring gland, (b) brain, (fb) fat body, (mi) midintestine, (sg) salivary gland, (gc) gastric caeca, (ed) eye imaginal disc, (as) anterior spiracle, (p) proventriculus, (g) ventral ganglion.