Table 3 PCR primers used to prepare pGL4-FABP7 promoter constructs
Position, cloning site and orientation | Sequence |
|---|---|
-1122 Bgl II Sense | CTTTCAGATCTGGTCAGCACTAGTAAG |
-468 Bgl II Sense | CACTAAGATCTCTCCTTTGTCTGCAAAG |
-351 Bgl II Sense | ATCTTAGATCTTTTCCTTGCAGTCTGAG |
-192 Bgl II Sense | GAACTAGATCTACTCCGCTAACCCAG |
-149 Bgl II Sense | CAAAGAGATCTGGAGCCTCACTCGAGC |
-83 Bgl II Sense | AGAGGAGATCTGGAGGGGTGTGTTTG |
-72 Bgl II Sense | GAGAGATCTGTTTGCAATTTAAATCACTGG |
-61 Bgl II Sense | TTTGAGATCTAAATCACTGGATTTTTGCCC |
-48 Bgl II Sense | TCACAGATCTTTTGCCCACCCTCTTTCC |
-36 Bgl II Sense | CCCAAGATCTTTCCAAATAAGAAGGCAG |
+89 Hin dIII Antisense | ACAGAAAGCTTCCACCATCCTTGCCC |