Table 2 RT-PCR and Q-PCR primers
cDNA | Sequence | Annealing temperature (°C) | Number of cycles |
|---|---|---|---|
FABP7 | TGACCAACAGTCAGAACTTT | 54 | 40 |
| Â | GCCATCCCATTTCTGTATGTG | Â | Â |
TBP | TTCGGAGAGTTCTGGGATTGTA | 58 | 40 |
| Â | TGGACTGTTCTTCACTCTTGGC | Â | Â |
BRN2 | CGGCGGATCAAACTGGGATTT | 57 | 40 |
| Â | TTGCGCTGCGATCTTGTCTAT | Â | Â |
OCT1 | GCATCCAACCACCAATTTGC | 57 | 30 |
| Â | GAGGTGAGGGTGATGCTTG | Â | Â |
OCT6 | GCGGCGCATCAAGCTGGG | 63 | 38 |
| Â | CGGTTGCAGAACCAGACGCG | Â | Â |
NFIA | CAGCCAAGTGACGCTGACA | 60 | 25 |
| Â | CCTCATTGCTCCTGGACTCAT | Â | Â |
NFIB (1) | CATAACCCAGGGAACTGGAG | 60 | 25 |
| Â | CTCTTTCGTGCCATGTTCGAC | Â | Â |
NFIB (2) | CTCCCATCTGTCTCACTCAGG | 58 | 25 |
| Â | CACGGGTGCTTCTTGCCAGTC | Â | Â |
NFIC | GGACAGGGATGGGCTCTG | 58 | 35 |
| Â | CGTTCTTCTGAGGCCAGTGC | Â | Â |
NFIX | GTTTTGTGACTTCCGGGGTCTG | 58 | 35 |
| Â | GGAAGGAGGGGAGGTGATG | Â | Â |
β-actin | CAAACATGATCTGGGTCATCTTCTC | 60 | 25 |
| Â | GCTCGTCGTCGACAACGGCTC | Â | Â |