Figure 8

Gel shift analysis with the MP probe. A) ³²P-labeled MP (Table 1 and Fig. 4) was added to HEK293 or RCC (ACHN, OS-RC-2, 769-P, 786-O, Caki-1, and TUHR14TKB) nuclear extracts. In (+) cell line, luciferase activity of -61+89 vector transfectant was increased above 30% than that of -48+89 vector transfectant. Arrowheads indicate significant band shifts. B) Competition analysis was performed using a variety of oligonucleotides (Table 1). A 100-fold excess of unlabeled competitor oligonucleotide, indicated in the figure, was added to each reaction. C) Five micrograms of HEK293 or TUHR14TKB nuclear extracts were incubated with 2 μg of the antibodies, as indicated in the figure, on ice for 30 min prior to addition of the ³²P-labeled oligonucleotide probes. The '*' indicates anti-NFI induced supershifts.