Figure 5

Gel shift analysis with the UP probe. A) ³²P-labeled UP (Table 1 and Figure 4) were added to HEK293 or RCC (Caki-1, OS-RC-2, TUHR14TKB, 769-P, 786-O, and ACHN) nuclear extracts. In (+) cell line, luciferase activity of -72+89 vector transfectant was increased above 30% than that of -61+89 vector transfectant. Arrowheads indicate significant band shifts. B) Competition analysis. Table 1 describes the oligonucleotide sequences. A 100-fold excess of unlabeled oligonucleotide indicated in the figure was added to each reaction. C) Five micrograms of HEK293 or ACHN nuclear extracts were incubated with 2 μg of antibody (see figure) on ice for 30 min prior to addition of the ³²P-labeled oligonucleotide probes. The '*' shows anti-BRN2 induced supershifts.