FABP7 promoter deletion analysis. HEK293 and RCC (TUHR14TKB, 786-O, 769-P, OS-RC-2, Caki-1, and ACHN) cells were cotransfected with the pGL4-FABP7 promoter constructs described above and the pGL4.74 plasmid control, and cultured for one day. Extracts prepared from transfected cells were assayed for luciferase activity. FABP7 promoter activities were normalized to the control's Renilla luciferase activity. The results shown are an average of at least three independent experiments with standard deviations indicated by the error bars. A: Deletion analysis of the FABP7 promoter region. B, and C: analyses of additional deletions.