Segmental RT-PCR scan of TRPC1 mRNA species. (a-c) PCR on reverse-transcribed human brain mRNA ('mRNA') and the cloned human Δ3 TRPC1 cDNA ('clone'). As indicated by the exon schematics below each gel, PCR primers (forward and reverse arrows) spanned from exons 1 to 5 (a), 6 to 10 (b), or 9 to 13 (c). ORF: open reading frame. (d) Example raw data from PCR primers spanning from exon 1 to exon 10. Sequencing showed that the upper band was full-length sequence except for exon 3 (Δ3) and that the lower band lacked exons 2, 3 and 5-9 (Δ 2,3,5-9). (a-d) Experiments were repeated independently 4 times and yielded similar results.