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Figure 3 | BMC Molecular Biology

Figure 3

From: Strand-specific transcriptome profiling with directly labeled RNA on genomic tiling microarrays

Figure 3

Strand-specific RT-PCR targeting P. gingivalis W83 genes. Antisense sequences targeted by RT-PCR: PG0279, PG0933, PG1069, PG0559, and PG0775 (marked A1-5, respectively). A3L and A5L were longer PCR products from PG1069 and PG0775. C1, PG1159 and C2, PG1144 were sense sequences targeted by RT-PCR. Columns from left to right: 1, RT-PCR amplicons on RNA duplicates for the targeted antisense and sense sequences; 2, PCR of the targeted antisense and sense sequences on gDNA; 3, RT-PCR of the targeted antisense sequences without reverse transcriptase; 4, RT-PCR amplicons on RNA duplicates targeting the mutB gene; 5, RT-PCR of mutB without reverse transcriptase.

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